A MODIFIED PERFUSION TECHNIQUE OF ISOLATED RAT LIVER

Isolated Perfusion of the Rat Liver

The isolated perfused rat liver (IPRL) has proven to be a useful model for the study qf physiology and pathology of the liver, For research in nonparenchymal cell (NPC) function that includes measurement of cytokine production (eg, TNF), it is necessary to have a sterilized perfusion system, We have modified the IPRL apparatus so as to be able to perform sterile perfusions of two livers simulta...

The Hepatoprotective Effects of Corn Silk against Dose-induced Injury of Ecstasy (MDMA) Using Isolated Rat Liver Perfusion System

Background: Corn silk (CS) is widely used in Iranian traditional medicine. The aim of this study was to investigate hepatoprotective activity of CS by Isolated Rat Liver Perfusion System (IRLP). Methods: Hydro-alcoholic extract of corn silk (10, 20, 40, and 100 mg kg-1) was evaluated for its hepatoprotective activity by IRLP. Phenol and flavonoid contents of the extract were determined as gal...

Digitonin perfusion of rat liver

Perfusing a rat liver with digitonin in the concentration range 2-20mg/ml results in complete decolorization of the organ within 45-250s. Decolorization progresses with time in the direction of flow, and it is therefore possible, by collecting the eluate, to obtain material from specific intracellular compartments of hepatocytes in different zones in the microcirculatory unit of the liver. The ...

A Technique for the Cannulation and Perfusion of Isolated Rat Epididymal Fat Pad.

An improved technique for isolated perfusion of rat livers and an evaluation of perfusates.

We have modified the apparatus for isolated rat liver perfusion (IPRL) in order to be able to perform two perfusions simultaneously. In addition, we studied the quality and stability of livers by comparison of five different perfusates: Blood (Group A), Original Krebs Henseleit buffer (Group B), Krebs buffer with glucose (Group C) or bovine serum albumin (BSA) added, (Group D). In a last group ...